Proteus bacteria in animal by-products and soil
https://doi.org/10.46845/1997-3071-2024-75-40-51
Abstract
The use of animal by-products as organic fertilizers is associated with the risk of environmental pollution by a wide range of various microorganisms, including sanitary-indicative bacteria of the genus Proteus spp. The article analyzed soil samples taken in an arable field fertilized by the neutralized liquid fraction of pig manure effluent (ME) pumped out of lagoons, as well as soil samples harvested at a distance of 500–600 m from the boundaries of the field. P. vulgaris (an indicator of soil contamination with organic substances of animal origin) and P. mirabilis (an indicator of fecal contamination) were detected in all samples. The amount of Proteus spp. in the arable land fertilized with ME turned out to be higher than in pig feces and fresh ME (immediately after separation), so the soil is a good habitat for bacteria of this genus. Territories under the influence of livestock complexes can be considered as places of reservation of opportunistic bacteria of the genus Proteus. The effect of various methods of processing the liquid fraction on the number of Proteus was evaluated in the laboratory. Various types of surfactants (cationic, anionic, nonionic), sodium hypochlorite, sulfuric acid (acidification to pH 5,5), as well as methods of aeration and creation of anaerobic conditions were used for the processing of liquid fraction. The observation time was 14 days. Samples of biofilms and bacterioplankton were used for microbiological research. The number of Proteus spp. in the biofilms of all experimental variants turned out to be 2–3 orders lower than in bacterioplankton. Aeration and anaerobic conditions had no effect on the abundance of Proteus spp. The use of nonionic surfactants and acidification reduced the number of Proteus spp. by 10 times, processing with NaОCl, cationic and anionic surfactants led to a decrease in the number of corresponding bacteria by 2 orders.
About the Authors
L. V. Pilip
Vyatka State Agrotechnological University
Russian Federation
Russian Federation
Larisa V. Pilip – PhD in Veterinary, Associate Professor
Kirov
N. V. Syrchina
Vyatka State University
Russian Federation
Russian Federation
Nadezhda V. Syrchina – PhD in Chemistry, Associate Professor, Senior Researcher
Kirov
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For citations:
Pilip L.V., Syrchina N.V. Proteus bacteria in animal by-products and soil. KSTU News. 2024;(75):40-51. (In Russ.) https://doi.org/10.46845/1997-3071-2024-75-40-51
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