Modulation of phagocytic activity of neutrophils with aqueous extracts of Arthrothamnus bifidus and Laminaria repens (Laminariales, Ochrophyta)

   Changes in nonspecific immune resistance of 25 persones before and after exposure on their blood in vitro by low-concentration priming solutions (LPS) obtained from aqueous extracts of Kamchatka’s laminariacean algae Arthrothamnus bifidus and Laminaria repens are discussed. Their blood samples have been activated by HPR twice: at the beginning of the experiment and after thirty days of daily oral using of algalgel obtained from Hedophyllum bongardianum. The immunomodulatory effect was assessed by the phagocytic activity of neutrophils, phagocytic number, absolute phagocytic index and the amount of phagocytosis. It has been shown that the substances contained in algalgel in an accessible form are able to soak from the gastrointestinal tract into the bloodstream and have a normalizing effect on the leukocyte profile of the blood. The LPS concentration of 0.25 % was ineffective; at the concentration of 5 % and higher, suppression of phagocytosis was observed. LPS 0.5, 1 and 2 %, especially from two-year-old samples of A. bifidus and L. repens, had the greatest priming effect on neutrophils and was complemented by inactivation of bacteria in the extracellular environment. LPS from L. repens induced extracellular degranulation processes, and those from A. bifidus led to the formation of neutrophil extracellular traps. The results of our study showed that the investigated species can be raw materials for the production of dietary supplements with an immunomodulating effect.

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