The influence of synthetic surfactants on the microbial composition of manure biofilm
https://doi.org/10.46845/1997-3071-2024-73-24-35
Abstract
The study of the composition, structure and communication between various microorganisms in biofilms is a priority direction of the development of environmental microbiology. Being a part of the biofilms bacteria, fungi, protozoa and other microorganisms become more resistant to the effects of adverse environmental factors. Microorganisms that form biofilms colonize various surfaces, as well as the skin and mucous membranes of animals and humans, acquire antibiotic resistance and resistance to disinfectants. The aim of the work was to assess the effect of various types of syn- thetic surfactants on the composition of biofilms formed on the surface of pig manure effluents. Surfactants are widely used in various industries, including industrial animal husbandry, mainly in the form of detergents and pharmacological preparations. Biofilms were removed from the surface of the manure effluents in compliance with the rules of asepsis and antiseptics 14 days after the start of the experiment. Microbiological studies have shown that the introduction of cationic surfactants, nonionic surfactants and anionactive surfactants leads to a significant transformation of the biofilms microbiota: the prevalence of fungi Aspergillus ssp. and Candida sp. (44.5–55.2%) over gram-positive microorganisms, characteristic of the control variant (81.49%), and the formation of denser biofilms, capable of reducing the processes of gas emission and odor load from manure effluents. Under the influence of surfactants from biofilms, Staphylococcus epidermidis, Lactobacillus ssp. disappeared from the composition of biofilms and Bifidobacterium ssp., and in the variant with anionactive surfactants also Bacteroides sp. and Pseudomonas spp. Clostridium ssp. showed the greatest resistance to surfactants.
About the Authors
L. V. Pilip
Vyatka State University
Russian Federation
Russian Federation
Larisa V. Pilip, PhD in Veterinary, Associate Professor of the Department of Zoological Hygiene, Physiology and Biochemistry
Kirov
N. V. Syrchina
Vyatka State Agrotechnological University
Russian Federation
Russian Federation
Nadezhda V. Syrchina, PhD in Chemistry, Associate Professor of the Department of Fundamental Chemistry and Chemistry Teaching Methods, Senior Researcher of the Laboratory of Biomonitoring of Vyatka State University and Komi of the research center of Ural division of the Russian Academy of Sciences
Kirov
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Review
For citations:
Pilip L.V., Syrchina N.V. The influence of synthetic surfactants on the microbial composition of manure biofilm. KSTU News. 2024;(73):24-35. (In Russ.) https://doi.org/10.46845/1997-3071-2024-73-24-35
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