Approaches to solving the problem of determing the sufficiency of marine placental collagen purification
https://doi.org/10.46845/1997-3071-2022-64-108-118
Abstract
Implementation of a unique method for producing marine placental collagen in the pilot operation conditions required a serious analysis of methods for assessing the parameters of individual technological stages. The main processes determining the quality of the finished product are separation of water-, salt- and alkali-soluble proteins in combination with the removal of lipids. To control the manufacturing process in the framework of in-process monitoring, it has been proposed to use an assessment of the dynamics of protein accumulation in extractants and the residual lipid content in the collagen. The analysis of standardized methods for determining the mass fractions of protein and fat used in food and pharmaceutical industries has shown a theoretical possibility of using the colorimetric method with a biuretic reagent and the gravimetric modified Soxhlet method, respectively, for technological purposes. Measurement of the protein content allowed for justification of a method for determining the duration of the extraction process under specific temperature conditions. The necessary and sufficient conditions are formulated as boundary factors in determining of this parameter. It has been proposed to consider the residual amounts of non-collagen proteins in the finished product as a technically difficult-to-remove impurity. The appliance of a widely used procedure for the quantitative determination of lipids with modern laboratory equipment suitable for production laboratories equipping, together with the analysis of other available standardized procedures, revealed a problem associated with the lack of an acceptable method due to the content of the measured component in the analyzed matrix at a level below reliable results. As a solution, it has been proposed to fix the value of remaining lipid content in product specification at the level of twice the repeatability limit of the most accurate available measurement procedure.
About the Authors
N. A. Rachkova
Kaliningrad State Technical University
Russian Federation
Russian Federation
Rachkova Natalya Anatolevna, 1 category engineer, Department of Chemistry
V. V. Soklakov
Kaliningrad State Technical University
Russian Federation
Russian Federation
Soklakov Vladimir Vladimirovich, PhD in Engineering, Associate Professor, Department of Food Products Technology
B. Y. Vorotnikov
Kaliningrad State Technical University
Russian Federation
Russian Federation
Vorotnikov Boris Yurievich, PhD in Engineering, Associate Professor; Head of the Department of Chemistry
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Review
For citations:
Rachkova N.A., Soklakov V.V., Vorotnikov B.Y. Approaches to solving the problem of determing the sufficiency of marine placental collagen purification. KSTU News. 2022;(64):108-118. (In Russ.) https://doi.org/10.46845/1997-3071-2022-64-108-118
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