Structure of the microbial community of the Verkhniy pond (Kaliningrad)
https://doi.org/10.46845/1997-3071-2023-71-24-37
Abstract
The formation of water quality in the reservoir and watercourses of the Verkhniy pond basin is caused by complex processes and is influenced by numerous natural and anthropogenic factors. The speed of metabolism and the short time of generation of microorganisms allow them to react to changing environmental conditions much faster than other components of biota. As a result of the analysis of the structure of the microbial community of the Verkhniy pond, heterogeneity of microbial populations has been revealed. Gram-negative bacteria of the families Enterobacteriaceae, Pseudomonadaceae, Vibrionaceae have been found in the composition of the bacterioflora of water. Gram-positive microflora included microorganisms of the genera Bacillus, Micrococcus, Staphylococcus. Gram-negative rod-like bacteria of the genus Pseudomonas, belonging to group 4 «Gram-negative, aerobic/micro-aerophilic rods and coccus», subgroup 4A, were widely distributed in microbial populations. Significant changes in microorganism communities (increase in the proportion of gram-negative bacteria) have been noted. The number of rod-like bacteria of the genus Aeromonas peaked in May 2023 (74.94 % of all isolated bacterial strains). The change in the structure of microbial communities towards an increase in the proportion of gram-negative bacteria indicates a pronounced anthropogenic pressure. The numbers of heterotrophic bacteria utilizing readily available organic matter in water ranged from 1,2х103 to 4,2х104 CFU/ml. The highest levels have been observed near the estuary of the Botanical Stream, indicating a high content of organic matter in the water. An increase in the number of heterotrophic bacterioplankton may be an indirect indicator of incomplete destruction, incomplete transformation of organic matter entering the watercourse, including anthropogenic origin. In the microflora of the water of the Verkhniy pond, the presence of sanitary-significant bacteria of the fecal group, which belong to the indicators of sanitary malaise of the aquatic environment has been detected.
About the Author
A. I. Moiseeva
Kaliningrad State Technical University
Russian Federation
Russian Federation
Anastasiya I. Moiseeva, Postgraduate student
Department of Aquatic Bioresources and Aquaculture
Kaliningrad
References
1. Bernikova T. A., Tsupikova N. A., Nagornova N. N. Rol' vodnykh ob"ektov v obespechenii ustoychivogo razvitiya gorodskoy sredy (na primere basseyna pruda Verkhnego v g. Kaliningrade) [The role of water bodies in ensuring sustainable development of the urban environment (on the example of the Verkhniy pond basin in Kaliningrad)]. Vestnik Rossiyskogo universiteta druzhby narodov. Seriya: Ekologiya i bezopasnost' zhiznedeyatel'nosti, 2013, no. 4, pp. 97–107.
2. Tsupikova N. A., Blotskaya E. N., Ryabtseva E. A. Nekotorye rezul'taty ekologicheskogo monitoringa pruda Verkhnego (g. Kaliningrad) v 2015 godu [Some results of environmental monitoring of the Verkhniy pond (Kaliningrad) in 2015]. Nauchno-issledovatel'skie publikatsii, 2016, no. 2, pp. 107–116.
3. Ryabtseva E. A., Tsupikova N. A. Prostranstvennoe raspredelenie gidrokhimicheskikh pokazateley v Verkhnem prudu goroda Kaliningrada [Spatial distribution of hydrochemical parameters in the Verkhniy pond (Kaliningrad)]. Izvestiya KGTU, 2019, no. 54, pp. 61–74.
4. Labinskaya A. S. [i dr.]. Obshchaya i sanitarnaya mikrobiologiya s tekhnikoy mikrobiologicheskikh issledovaniy : uchebnoe posobie [General and sanitary microbiology with microbiological research techniques : textbook]. Saint-Petersburg, Lan’, 2022, 588 p.
5. Tsupikova N. A., Bugranova O. S. Vliyanie samoochishcheniya na kachestvo vody v sisteme prudov Verkhniy – Nizhniy (g. Kaliningrad) letom 2015 g. [Influence of self-purification on water quality in the Verkhniy – Nizhniy pond system (Kaliningrad) in the summer of 2015]. Prirodnye resursy, ikh sovremennoe sostoyanie, okhrana, promyslovoe i tekhnicheskoe ispol'zovanie : materialy X Vserossiyskoy nauchno-prakticheskoy konferentsii (20–22 marta 2018 g.) [Natural resources, their current condition, protection, industrial and technical use : proceedings of the IX National Russian Scientific and Practical Conference (20-22 March 2018)]. Petropavlovsk-Kamchatskiy, KamchatGTU, 2018, pp. 95–98.
6. Sakharova O. V., Sakharova T. G. Vodnaya mikrobiologiya : uchebnik dlya vuzov [Water microbiology : textbook for universities]. Saint-Petersburg, Lan’, 2022, 260 p.
7. Gosmanov R. G., Ibragimova A. I., Galiullin A. K. Mikrobiologiya i immunologiya : uchebnoe posobie [Microbiology and immunology : textbook]. Saint-Petersburg, Lan’, 2023, 240 p.
8. Tekanova E. V., Makarova E. M., Kalinkina N. M. Otsenka sostoyaniya vody pritokov Onezhskogo ozera v usloviyakh antropogennogo vozdeystviya po mikrobiologicheskim i toksikologicheskim pokazatelyam [Assessment of the condition of the water of lake Onego inflowing streams under human impact using microbiological and toxicological parameters]. Trudy Karel'skogo nauchnogo tsentra RAN, 2015, no. 9, pp. 44–52.
9. Samoylova I. Yu., Anganova E. V., Savilov E. D. Taksonomicheskaya kharakteristika mikrobiotsenoza reki Leny v rayone g. Yakutska, Khangalasskogo i Namskogo rayonov (Respublika Sakha (Yakutiya)) [Taksonomic features of microbiocenosis of the river Lena in the region of Yakutsk, Hangalassky and Namsky areas (Republic Sakha (Yakutia))]. Sibirskiy meditsinskiy zhurnal, 2009, no. 7, pp. 192–194.
10. Kazimirchenko O. V., Kotlyarchuk M. Yu. Praktikum po mikrobiologii : uchebnoe posobie [Microbiology Workshop : textbook]. Saint-Petersburg, Lan’, 2020, 124 p.
11. Gerkhardt F. Metody obshchey bakteriologii [Methods of general bacteriology]. Moscow, Mir, 1984, vol. 3, 264 p.
12. Khoult Dzh., Krig N., Snit P., Steyli Dzh., Uil'yams S. Opredelitel' bakteriy Berdzhi [Bergey's bacteria identifier]. Moscow, Mir, 1997, vol. 1, 432 p.
13. Khoult Dzh., Krig N., Snit P., Steyli Dzh., Uil'yams S. Opredelitel' bakteriy Berdzhi [Bergey's bacteria identifier]. Moscow, Mir, 1997, vol. 2, 368 p.
14. Bogdanova O. Yu. Mikrobiologiya vodnykh ekosistem : uchebnoe posobie [Microbiology of aquatic ecosystems : textbook]. Murmansk, FGBOU VPO «MSTU» Publ., 2016, 150 p.
15. Tsupikova N. A., Men'shenin A. S. Otsenka sostoyaniya pruda Shenfliz (g. Kaliningrad) po gidrokhimicheskim pokazatelyam v 2018 godu [Assessment of the Shenfliz pond condition (Kaliningrad) due to hydrochemical indicators in 2018]. Prirodnye resursy, ikh sovremennoe sostoyanie, okhrana, promyslovoe i tekhnicheskoe ispol'zovanie : materialy IX Vserossiyskoy nauchno-prakticheskoy konferentsii (19–21 marta 2019 g.) [Natural resources, their current condition, protection, industrial and technical use : proceedings of the IX National Russian Scientific and Practical Conference (19-21 March 2019)]. Petropavlovsk-Kamchatskiy, KamchatGTU, 2019, pp. 128–132.
16. Sevost'yanova E. A. [i dr.] Sovremennoe ekologicheskoe sostoyanie vodoemov rekreatsionnogo naznacheniya Kaliningrada [Current ecological condition of recreational water bodies in Kaliningrad]. Prirodnye resursy, ikh sovremennoe sostoyanie, okhrana, promyslovoe i tekhnicheskoe ispol'zovanie : materialy XIII Vserossiyskoy nauchno-prakticheskoy konferentsii (29–30 marta 2022 g.) [Natural resources, their current condition, protection, industrial and technical use : proceedings of the XIII National Russian Scientific and Practical Conference (29-30 March 2022)]. Petropavlovsk-Kamchatskiy, KamchatGTU, 2022, pp. 193–197.
17. Lozitskaya E. A., Tsupikova N. A. Nekotorye rezul'taty ekologicheskogo monitoringa pruda Pelavskogo v 2015–2016 gg. [Some results of environmental monitoring of the Pelavsky pond in 2015–2016]. Vestnik molodezhnoy nauki, 2017, no. 1, pp. 1–7.
18. Lozitskaya E. A., Bugranova O. S., Kazimirchenko O. V., Tsupikova N. A. Otsenka ekologicheskogo sostoyaniya ozera Karpovskogo v 2019 g. [Assessment of the ecological state of Lake Karpovsky in 2019]. Izvestiya KGTU, 2020, no. 59, pp. 73–87.
19. Morsy K., Mishra A., Galal M. Water Quality Assessment of the Nile Delta Lagoons. Air, Soil and Water Research, 2020, vol. 13, pp. 1–11.
Review
For citations:
Moiseeva A.I. Structure of the microbial community of the Verkhniy pond (Kaliningrad). KSTU News. 2023;(71):24-37. (In Russ.) https://doi.org/10.46845/1997-3071-2023-71-24-37
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