Technological aspects of improving the efficiency of phytocomposition based on sea buckthorn meal flavonoids

Currently, the multi-tonnage waste – meal – remaining after processing the fruits of buckthorn buckthorn (Hippophae rhamnoides L.) does not find industrial use, despite the macro- and micro-elements and other biologically active substances contained in it, in particular – flavonoids. The composition of the meal determines its high biological value and the possibility of obtaining a pharmaceutical phytocomposition of anti-inflammatory action, which is important and timely in the current COVID-19 situation. Along with this, 80% of the world's population consider herbal medicines as an alternative to synthetic drugs, thanks to the well-known list of their properties. However, only a small part of polyphenols is absorbed in the small intestine, and the large part reaches the colon area without being absorbed. Therefore, an effective system of their delivery is required to achieve a therapeutic effect. To solve the tasks set in the work, a method of micronization of the purified flavonoid fraction of sea buckthorn meal has been developed. A rational mode of ultrasonic micronization has been determined: processing for 10 minutes at a power of 50 watts, during which the smallest particle size has been obtained – 1400 nm or less. The micronized sample is a homogeneous fine powder of brown-yellow color, neutral taste, odorless and foreign impurities, with a base substance content of 96 %, a moisture content of about 5 %, ash content of 0,04 %, toxic elements and extraneous microflora have not been detected, which indicates not only the high quality of the sample obtained, but also the manifested antibacterial effects of phenolic substances and ultrasonic treatment. Analysis of the micronization degree and the effectiveness of biological action has been confirmed by the example of antioxidant activity and phlebotropic action, indirectly proving P-vitamin activity. The micronization method provides a 4,5-fold reduction in particle size, an increase in prooxidant activity on the model of reduction of sulfhydryl groups of glutathione with a constant chemical composition of the complex.

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